The postal sorting office in Great James Street.
Staff had refused to enter the Great James Street office on Tuesday due to concerns over the spread of Covid-19.
Almost 50 staff are off work because of Covid-19 at the site, the Communication Workers’ Union (CWU) said.
Royal Mail confirmed the clean went ahead on Tuesday and caused “minor disruption to service”.
It added that it was “working to resolve any additional concerns with our colleagues and our union”.
The CWU’s Charlie Kelly said around 50 workers are currently absent because of Covid-19 at the site.
He said staff returned to the office on Wednesday morning and were working to address a backlog of mail.
“At the minute we have 50 staff down plus six on annual leave, that’s 56 staff out of 120 staff. With those numbers off it is going to take a while to clear up any backlog,” he said.
A further clean of Royal Mail vehicles at the site is to take place later on Tuesday, he added.
Royal Mail staff in stand off at postal office in Derry on Tuesday
There have been 4,439 confirmed cases in the Derry and Strabane council area since March – 3,156 of them were diagnosed since the start of October.
On Tuesday, Stormont’s chief scientific adviser said there was “strong evidence” that Coronavirus restrictions imposed in the Derry City and Strabane council area are making a difference.
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